See what picklet can do
picklet opens a sequence file, shows what is inside it, and saves the parts you pick as FASTA.
The screenshots below show the main things it does:
- Open files in about twenty formats
- Read the format from the content, not the file name
- Pick sequences across several files at once
- Filter a long list by length, name, type or GC
- Translate picked DNA to protein
- Drop duplicates and set the output order
Files are processed in the browser, on your own machine — nothing is sent over the network.
Open files of different formats togetherA GenBank export saved as .txt, a Sanger .ab1 and a multi-record FASTA, open side by side in one list.
The format comes from the content, so the misnamed file is still read as GenBank, and the records ticked across all three become one FASTA on the right.
Narrow a long list with filtersFilters combine to hide what you are not after: here a length range and a GC range leave four of the eleven coding sequences of a phage genome.
What is hidden cannot be picked, so it never reaches the saved file.
Translate picked DNA to proteinThe picked coding sequences are translated on the way out, in a chosen reading frame and with any of the NCBI genetic codes.
The preview shows the protein that will be saved; the source file is left alone.
Drop duplicates and set the orderTwo files that share four sequences are merged into one FASTA: the repeats are collapsed, with a count of how many went, and the records come out sorted by length.
Duplicates are judged on the final sequence, after the transformations above.