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View, compare and inspect Sanger / .ab1 chromatograms
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2×U6 gRNA — read 1
Sanger trace of a 2×U6-driven gRNA (CRISPR) construct — 987 bp, ABI 3730. Has both raw and analyzed traces, so it shows off the single-file raw-vs-analyzed view.
2×U6 gRNA — read 2
A second Sanger read of the same 2×U6 gRNA construct — 1041 bp, ABI 3730. Open it next to “2×U6 gRNA — read 1” in Two files to try the diff.
Hand-edited read
A Sanger read (ABI 3500) whose basecalls were manually curated — it carries both the software calls (PBAS2, 413 bp) and a hand-edited version (PBAS1, 376 bp). Open it to try the Called/Edited toggle and step through the edits.
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